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Ouchi, Kazuki
Hosha Kagaku, (49), p.3 - 7, 2024/03
I introduce the elucidation of the deposition following the oxidation state of uranium and the electrochemical behavior of uranium(IV) chloride in an ionic liquid-organic mixture, as a basic study of in-solution reactions. In addition, I introduce the development of separation methods for actinides using a microchemical chip and polyacrylamide gel electrophoresis, as an applied study for quantitative analytical methods for small amounts of samples.
Haraga, Tomoko; Saito, Shingo*
Bunseki Kagaku, 70(12), p.671 - 679, 2021/12
We developed highly sensitive capillary electrophoresis-laser-induced fluorescence detection methods for lanthanide (Ln) and actinide (An) ions with small sample volume and low emission of waste, by which the radiation risk can be minimized. Specifically, determination of Nd ion in spent nuclear fuel, effective separation between Am and Cm ion, and specific detection of UO
in real radioactive samples were achieved by molecular design of fluorescence probes composed of an aminocarboxylate chelating moiety, a fluorophore and a spacer, and unique separation mode based on dynamic ternary complexation. We found that there are appropriate combination of probe and ternary complexation for detection and separation of each Ln and An ions. For example, acyclic and macrocyclic hexadentate is suitable for Ln
, Am and Cm, and planer tetradentate with 
electron system is specific for UO, with ppt-sub ppt level detection.
Haraga, Tomoko; Tsujimura, Hiroto*; Miyauchi, Saori*; Kamimura, Takuya*; Shibukawa, Masami*; Saito, Shingo*
Electrophoresis, 41(13-14), p.1152 - 1159, 2020/07
Times Cited Count：7 Percentile：44.04(Biochemical Research Methods)A novel combination of CE-based separation techniques was used for the precise fractionation of ionic compounds from impurities. The combination of on-capillary concentration and separation using transient isotachophoresis, with multiple injections and a two-point detection system provided higher efficiency, and accuracy at a microliter-scale injection volume, than when CE was individually used for purification. In this paper, we present successful applications of the CE fractionation techniques for the purification of fluorescein, fluorescein-4-isothiocyanate, two fluorescent metal ion probes, and a fluorescein-modified DNA aptamer. The purity of the isolated fluorescent probes ranged from 95 to 99%. The purified probe solutions were practical for use as purified stock solutions. The method developed was useful for the purification of anionic fluorescent reagents to be of ultratrace analytical grade for use with CE-LIF.
Ogino, Masataka*; Owaki, Eiji*; Shirase, Mitsuyasu*; Nakayama, Masashi
Konkurito Kogaku Nenji Rombunshu (DVD-ROM), 39(1), p.703 - 708, 2017/07
no abstracts in English
raysYokota, Yuichiro; Shikazono, Naoya; Tanaka, Atsushi; Hase, Yoshihiro; Funayama, Tomoo; Wada, Seiichi; Inoue, Masayoshi*
Radiation Research, 163(5), p.520 - 525, 2005/05
Times Cited Count：20 Percentile：50.54(Biology)Higher plants are generally more tolerant to ionizing radiation than mammals. To explore the radiation tolerance of higher plants, the amount of DNA double-strand breaks (DSBs) induced by -rays was investigated in tobacco BY-2 cells and compared with that investigated in Chinese hamster ovary (CHO)-K1 cells as a reference. The resulting DNA fragments were separated by pulsed-field gel electrophoresis and stained with SYBR Green I. Initial DSB yield was then quantified from the fraction of DNA fragments shorter than 1.6 Mbp based on the assumption of random distribution of DSBs. The DSB yield in tobacco BY-2 cells (2.0 
0.1 DSBs Gbp
Gy) was only one-third of that in CHO-K1 cells. Furthermore, the calculated number of DSBs per diploid cell irradiated with -rays of mean lethal dose was five times greater in tobacco BY-2 cells (263.2 13.2) than in CHO-K1 cells. These results suggest that the radiation tolerance of tobacco BY-2 cells appears to be due to not only a lower induction of DNA damage but also a more efficient repair of the induced DNA damage.
by pulsed-field gel electrophoresisKikuchi, Masahiro; Narumi, Issei; Kobayashi, Yasuhiko
JAERI-Conf 2002-005, P. 185, 2002/03
The most striking feature of the radioresistant bacterium 
is that it can mend over 100 double-strand breaks of genomic DNA during post-irradiation incubation. This process can be clearly visualized using pulsed-field gel electrophoresis (PFGE). By a combination of protein synthesis inhibition treatment and PFGE analysis, it was possible to estimate an initial period required for induction of DNA repair proteins (induction time) and a total period required for completing DNA repair (repair time). PFGE is a powerful tool to analyze DNA damage and its repair process.
*; Ito, Hitoshi; *; Ishigaki, Isao; *
Shokuhin Shosha, 25(1-2), p.83 - 88, 1990/00
no abstracts in English
Ogino, Masataka*; Owaki, Eiji*; Shirase, Mitsuyasu*; Nakayama, Masashi
no journal, ,
no abstracts in English
Haraga, Tomoko; Marumo, Kazuki*; Saito, Takumi*; Shibukawa, Masami*; Saito, Shingo*
no journal, ,
no abstracts in English
Horiguchi, Naoki
no journal, ,
This presentation explains the overview of a numerical simulation for a lithium (Li)-7 enrichment technology using a multi-channel counter-current electrophoresis (MCCCE) method. Concretely, it explains the numerical simulation method based on a computational fluid dynamics code, TPFIT, and a commercial software for electric field analysis and the numerical simulation result of Li ion behavior in a single channel of a Li-7 enrichment experimental apparatus using the MCCCE method.
Ouchi, Kazuki
no journal, ,
I describe on the elucidation of the deposition following the oxidation state of uranium and the electrochemical behavior of uranium(IV) chloride in an ionic liquid-organic mixture, as a basic study of in-solution reactions. In addition, I describe on the development of separation methods for actinides using a microchemical chip and polyacrylamide gel electrophoresis, as an applied study for quantitative analytical methods for small amounts of samples.
